| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Meneses Kovacs, Athena M (12)
|
FGSFTL |
28.59 |
28.56 |
-0.03 |
| 2 |
Balfour, Lauren A (12)
|
FGUN-0 |
30.87 |
31.65 |
0.78 |
| 3 |
Egizi, Bella M (11)
|
FGPCS |
35.96 |
33.80 |
-2.16 |
| 4 |
Kuhn, Emma A (12)
|
ZZSEAL |
33.20 |
34.16 |
0.96 |
| 5 |
Kraft, Cassandra J (12)
|
FGGRSC |
32.83 |
34.28 |
1.45 |
| 6 |
Saporta, Camila (12)
|
FGGRSC |
35.41 |
34.55 |
-0.86 |
| 7 |
Arps, Kassie G (12)
|
GASA |
36.77 |
34.66 |
-2.11 |
| 8 |
Sell, Madeline M (12)
|
ILFOX |
37.89 |
34.69 |
-3.20 |
| 9 |
Dinehart, Elena S (12)
|
FGAKS |
34.78 |
34.72 |
-0.06 |
| 10 |
Levet, Charlotte (12)
|
FGNPB |
36.02 |
34.78 |
-1.24 |
| 11 |
Barnhardt, Rylie D (12)
|
FGSFTL |
35.00 |
35.26 |
0.26 |
| 12 |
Armbruster, Kaitlyn P (12)
|
FGPCS |
34.04 |
35.51 |
1.47 |
| 13 |
Velasco, Valentina (12)
|
FGGRSC |
37.99 |
35.87 |
-2.12 |
| 14 |
Smutny, Madeline R (12)
|
FGAKS |
37.00 |
35.96 |
-1.04 |
| 15 |
Portik-Gergely, Kata (11)
|
FGPCS |
37.43 |
36.35 |
-1.08 |
| 16 |
Arfa, Angelique F (11)
|
FGPCS |
38.31 |
37.44 |
-0.87 |
| 17 |
Udell, Stephanie R (11)
|
FGSFTL |
38.14 |
37.53 |
-0.61 |
| 18 |
Leland, Gretchen P (12)
|
ILFOX |
32.26 |
37.88 |
5.62 |
| 19 |
Norton, Hannah M (11)
|
FGUN-0 |
40.57 |
38.08 |
-2.49 |
| 20 |
Huffine, Emily G (12)
|
ILFOX |
37.65 |
38.26 |
0.61 |
| 21 |
Lin, Sara S (11)
|
FGUN-0 |
36.45 |
38.42 |
1.97 |
| 22 |
Runk, Sydney M (12)
|
FGNPB |
40.96 |
38.86 |
-2.10 |
| 23 |
Heilpern, Meghan L (12)
|
FGNPB |
39.57 |
39.22 |
-0.35 |
| 24 |
Walker, Syven N (12)
|
FGSUN |
41.29 |
41.35 |
0.06 |
| 25 |
Coan, Maya O (12)
|
FGSFTL |
34.34 |
41.40 |
7.06 |
| 26 |
Kuncas, Holly L (12)
|
FGSFTL |
41.19 |
42.13 |
0.94 |
| 27 |
Zumbrum, Alana L (11)
|
FGSFTL |
41.19 |
42.35 |
1.16 |
| 28 |
Genz, Cassandra N (11)
|
FGBRST |
41.29 |
50.43 |
9.14 |
|
Botting, Riley E (12)
|
FGUN-0 |
38.42 |
DQ |
- |
|
Leete, Siera A (12)
|
FGSFTL |
41.02 |
NS |
- |
|
Chandler, Eliza J (12)
|
FGMS |
35.69 |
NS |
- |
|
Hamann, Stella R (11)
|
FGGRSC |
41.25 |
NS |
- |